 |
2004
OMIG, Abstract 20
OMIG
Main Page | 2004
Abstracts | < Previous
| Next >
Efficacy
of Intrastromal Corneal Injection of Natamycin on Experimental Fusarium
solani Keratitis in Rabbits
L Barbosa de Sousa, AI Shiguematsu, AS Schellini, OF Gompertz, E
Bagagli, CA Padovani. Department of Ophthalmology, Federal University
of Sao Paulo, Sao Paulo, Brazil
Purpose:
To evaluate the efficacy of intrastromal corneal injection of natamycin
on experimentally-induced Fusarium solani keratitis in
rabbits.
Methods: Fusarium solani keratitis was
induced on the right eye of 45 male Norfolk rabbits following Ishibashi's
technique (1978). Seven days after fungal inoculation, the rabbits
were divided, by draw, into 3 groups of 15 animals each: Group I
(GI): treated with a single 40 ul intrastromal corneal injection
of 5% natamycin suspension in 2% methylcellulose; Group II (GII):
treated with 5% natamycin drops, hourly, for 7 days; and Group III
(GIII): treated with a single 40 ul intrastromal corneal injection
of 2% methylcellulose. The drug injections will be made through
two 250 um deep intrastromal tunnels (20 ul into each) created by
using a double Ferrara's spatula for intrastromal corneal ring segments.
Seven days after treatment, all animals were sacrificed and their
infected corneas were trephinated, excised, thoroughly washed with
sterile saline solution, macerated and embedded individually into
10 ml of BHI (brain-heart infusion) broth with chloramphenicol and
gentamicin. Ten ul samples from these suspensions were seeded on
Sabouraud's agar plates every 24 hours until the 7th
day after sacrifice. The number of colony-forming units (CFU) was
counted and data was submited to statistical analysis.
Results: Nonparametric analysis of repeated measures
showed statistically significant differences between the groups
on days 3 (GI<GII<GIII, p<0.005), 4 (GI<GII=GIII, p<0.01)
and 6 (GI=GII<GIII,
p<0.05).
Conclusion: A single intrastromal corneal injection
of natamycin appears to be as efficacious as hourly administration
of natamycin drops for 7 days on experimentally-induced Fusarium
solani keratitis in rabbits.
OMIG
Main Page | 2004
Abstracts | < Previous
| Next >
|
|
|
