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2005 OMIG, Abstract 16

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The Eyelid Cleansers "EYECL08" and "EYECL09" are Bactericidal for 40 Endophthalmitis Organisms.

Jeffrey P. Gilbard, MD; Barbara Paton, BS, MT; Massachusetts Eye and Ear Infirmary, Harvard Medical School, Advanced Vision Research, Boston, MA

PURPOSE: Enophthalmitis is a devistating complication of eye surgery, and the eyelids are frequently the source of the offending organisms. The antibacterial action of eyelid cleansers EYECL08 and EYECL12 was tested.

METHODS: Using stock cultures of Pseudomonas aeruginosa (PA), Moraxella caterrhalis (MC), E. coli (EC), Serratia marcescens (SM), and Staph aureus (SA), zone diffusion assays were performed. We then collected 40 organisms isolated from cases of enophthalmitis that had presented to the Massachusetts Eye & Ear Infirmary for broth testing. The organisms comprised 19 strains of S. epidermidis, 8 strains of SA, 4 strains of PA, 3 strains of methicillin-resistant SA, 3 strains of S. warneri, and 3 strains of SM. Inoculums were prepared from overnight cultures grown on inhibitory media. 4 or 5 well isolated colonies of each isolate were emulsified in 5 ml of sterile distilled water. The solutions were adjusted to approximate a 0.5 McFarland turbidity standard and then10 ul aliquots were inoculated into 1) a control tube containing 1 ml oftrypicsoy broth, 2) 1 ml of EYECL08 and 3) 1 ml of EYECL12. Tubes were incubated overnight in a non-C02 incubator.

RESULTS: Zone diffusion assays showed inhibition zones against PA, MC EC, SM and SA. In broth testing all controls tubes showed growth. EYECL08 and EYECL12 tubes showed no growth.

CONCLUSIONS: EYECL08 and EYECL12, eyelid cleansers dispensed as a foam for convinient patient use in the days leading upto eye surgery, are bactericidal and may help reduce the incidence of endophthalmitis.

Disclosure code: E,P

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