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2009 OMIG, Abstract 12

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The effect of bacterial proliferation to lens capsule.
S. Kobayakawa, T. Tochikubo
Department of Ophthalmology, Toho U., Tokyo, Japan.

Purpose: To investigate the effect of bacterial proliferation to the lens capsule in removed pig eyes (ex vivo).
Methods: Staphylococcus epidermidis, ATCC 35984 (biofilm-producer) and methicillin-resistant Staphylococcus aureus (MRSA), UA-1 (clinically isolated strain) were used. Small quantities of the culture were subcultured on the BHI broth overnight at 37°C. Next, cultures were kept frozen at -75°C in MicrobankTM (PRO-LAB Diagnostics, Toronto, Canada ). Phacoemulsification were performed in fresh removed pig eyes, the incision size was increased to approximately 5–6 mm. Two beads from MicrobankTM  tube (bacteria were attached to each bead) were inserted into the capsular bag for pig eyes. Wound closure was achieved with a 8-0 Vicryl suture, 0.1–0.2 ml of the BHI broth was administered in the anterior chamber of the surgical pig eyes at the end of surgery. Those surgical pig eyes were kept in a sterile 50ml plastic centrifuge tubes with 10ml PBS at 37°C. After incubation for 24 and 48 hours, those eyes were fixed with 10% formalin, observations were performed with hematoxylin-eosin stain.
Results: The destruction of ocular tissue was ongoing at 48 hours of incubation. As for the ATCC 35984 strain, those bacteria were aggregated on the surface of anterior and posterior lens capsule at 24 hours of incubation. As for the MRSA (UA-1) strain, bacteria were infiltrated into the anterior and posterior lens capsule.
Conclusions: It was possible to investigate the effect of bacterial proliferation to the lens capsule using by removed pig eyes. Pathological changes were noted in the lens capsules of eyes infected with MRSA at 24 hours incubation.
Disclosure Code; N

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