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2010 OMIG, Abstract 14

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Intrastromal versus topical moxifloxacin in a rabbit model of Pseudomonas aeruginosa keratitis
T. Chan, J.I. Stuart, L. Allen, R. Mather, Ivey Eye Institute, University of Western Ontario, London, Ontario, Canada

Purpose:  To compare the antibacterial effect of moxifloxacin administered as a single intrastromal corneal injection to topically administered moxifloxacin in the treatment of Pseudomonas aeruginosa (PA) keratitis in a rabbit model.
Methods: Each eye of 24 New Zealand White rabbits was intrastromally inoculated with ~106 colony-forming units of PA.  16 hours later, 6 rabbits were euthanized to determine the number of corneal bacterial colonies at the onset of therapy. The remaining 18 rabbits were divided equally into 3 groups: 1) intrastromal moxifloxacin, 2) topical moxifloxacin, and 3) topical saline control. For group 1, a single intrastromal dose of 0.05 cc moxifloxacin was injected around the infiltrate of each cornea.  For groups 2 and 3, one drop was applied every 15 minutes for 5 doses then every 30 minutes for 14 doses per eye.  9 hours after the initiation of treatments, all animals were euthanized and corneas were harvested. Colony counts from all corneas were determined.
Results: All corneas had significant infiltrate prior to onset of therapy, but only those from the topical saline group showed persistent infiltrates at the end. After the treatment period, the intrastromal and topical moxifloxacin groups had significantly lower colony counts than saline control (P<0.05, ANOVA).  There was no significant difference between the colony counts of the intrastromal and topical moxifloxacin groups.
Conclusions: Intrastromal and topical moxifloxacin showed equivalent efficacy against P. aeruginosa in a rabbit keratitis model. Intrastromal antibiotic injection may be an efficacious alternative to around-the-clock drops in treating bacterial keratitis.

Disclosure Code: Financial support, University of Western Ontario Pilot Research Fund

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