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2012 Agenda and Abstracts | < Previous | Next >

2012 OMIG Abstract 2

The Effect of the Drug Released Soft Contact Lens that Releases
Antibiotics in a Sustained Manner
S. Kobayakawa1, T. Matsunaga2, A. Kanayama1, Y. Yamazaki2, T. Sato2, I. Kobayashi1, T. Tochikubo1
1Toho University; 2Seed Co., Ltd., Tokyo, Japan

Purpose:  We developed a drug released soft contact lens (DR-SCL) of a hydrogel material capable of releasing antibiotics in a sustained manner. The purpose of this study was to investigate the preventive effect of the DR-SCL to bacterial endophthalmitis in a rabbit model.  

Method:  Gatifloxacin (GFLX) 0.3% eye drops were used. Staphylococcus aureus (MRSA, ATCC 43300) was used to induce experimental endophthalmitis. Each DR-SCL was soaked in GFLX 0.3% eye drop solutions and uptake of the antibiotic using an ion ligand mechanism. Forty-two Japanese albino rabbits were divided into three groups post-inoculation of S.aureus to anterior chamber. DR-SCL group, SCLs presoaked with GFLX were administered to eyes (n=15); SCL group, SCLs without antibiotics administered to eyes (n=15); eye drop group, topical GFLX was administered to eyes twice or three times every four hours for three days (n=12). At 24, 48, and 72 hr post-inoculation, three rabbits from each group were euthanized, and those inoculation eyes enucleated for the enumeration of bacteria. Also, slit-lamp examination (SLE) was performed at each timepoint.

Results:  The bacterial populations were significant differences among  DR-SCL groups (0-3 log10CFU/ml), SCL group (4.82-7.3 log10CFU/ml) and eye drop group (3.54-4.17 log10CFU/ml) through 72 hours, respectively (p<0.05). There were no bacteria in the DR-SCL group eyes at 24 and 48 hours post-inoculation. However, in the eye drops group, bacterial populations were 104 CFU/ml at 24 and 48 hours.

Conclusions: DR-SCL contained with GFLX prevented to the growth of S.aureus through 72 hours. It was suggested that DR-SCL have a preventive effect against acute postoperative endophthalmitis.

Disclosure: None

2012 Agenda and Abstracts | < Previous | Next >