The Charles T. Campbell Eye Microbiology Lab
UPMC | University of Pittsburgh Medical CenterUniversity of Pittsburgh Schools of the Health Sciences
HomeAbout UsLab Diagnostic TestingAntibiotic SusceptibilityAntimicrobial TherapyCurrent ResearchContact Us

2004 OMIG, Abstract 9

OMIG Main Page | 2004 Abstracts | < Previous | Next >

Comparison of In-Vitro Sensitivity Rates of the Ophthalmic Fluoroquinolones and Vancomycin Against Propionibacterium acnes Ocular Isolates
M Shah, D Ritterband, TL Kajura, W Perez, D Shapiro, JA Seedor, R Koplin. New York Eye and Ear Infirmary Department of Ophthalmology, New York, New York

Purpose: To study the in-vitro susceptibility profiles of the ophthalmic fluoroquinolone agents and vancomycin against Propionibacterium acnes (P. acnes), a frequent cause of post-cataract chronic endophthalmitis.
Methods: 42 P. acnes ocular isolates archived at the New York Eye and Ear Infirmary (NYEEI) from cases of corneal ulcer (24), chronic endophthalmitis (12), corneal rim culture (7), and an infected scleral buckle (1) were tested for in vitro susceptibility against vancomycin, moxifloxacin, gatifloxacin, levofloxacin, ofloxacin and ciprofloxacin. P. acnes isolates were subcultured on Brucella Agar - 5% sheeps-blood fortified with Vitamin K and Hemin. The subcultures was inoculated into anaerobic broth to make a 1.0 McFarland turbidity. The suspension was vortexed and replated on the Brucella media. Each culture was incubated at 35° C for 24-48 hours in anaerobic conditions. E-test strips were used to generate MIC values for ciprofloxacin, ofloxacin, levofloxacin, gatifioxacin, moxifloxacin, and vancomycin.
Results: 40 P. acnes isolates were sensitive to all antibiotics. Two P. acnes isolates (1 ulcer, 1 rim) were resistant to all flouroquinolone (FQ) agents but sensitive to vancomycin. The mean MIC for 40 P. acnes isolates sensitive to the (FQ) agents was in descending order ofloxacin (.87), ciprofloxacin (.45), levofloxacin(.35), gatifloxacin (.16), moxifloxacin (.l4). The mean MIC for the 40 FQ-sensitive P.acnes isolates for vancomycin was (.97).
Conclusions: The fourth generation fluoroquinolone agents moxifloxacin and gatifloxacin demonstrate excellent in vitro sensitivity profiles with low MIC's against almost all P. acnes isolates. The potential use of the moxifloxacin or gatifloxacin in the intraocular treatment of these organisms warrants further study.

Disclosure code: F

OMIG Main Page | 2004 Abstracts | < Previous | Next >

Top of Page

Web Site Terms of Use | E-mail Terms of Use | Medical Advice Disclaimer
UPMC | Affiliated with the University of Pittsburgh Schools of the Health Sciences | Contact UPMC