Lab Diagnostic Testing: Cytology

Microscopic examination of direct ocular specimens may provide valuable information but depend on the quality of the sample. Smears are generally obtained from the conjunctiva, cornea, aqueous, vitreous, and punctum. There are many types of stains used to examine direct specimens. The gram and giemsa stains are used in our laboratory. Gram stain is used to examine for bacteria, and the giemsa stain is used to determine cytology. The giemsa stain is also excellent for detecting bacteria, fungus, and Acanthamoeba. Many laboratories are uncomfortable with the giemsa stain because of limited experience. Laboratories may provide the same answers with other staining techniques.

Conjunctival specimens
Corneal specimens
Aqueous and vitreous specimens

Corneal specimens
The staining of corneal specimens is a valuable diagnostic tool that can guide crucial keratitis therapy whether to pinpoint or rule out infection. The presence of bacteria, fungus, and Acanthamoeba can commence earlier therapy. An adequate corneal specimen is a function of the experience of the presiding ophthalmologist. ONLY TRAINED OPHTHALMOLOGISTS SHOULD OBTAIN CORNEAL SPECIMENS. Corneal specimens should be taken with a spatula, surgical blade, or jeweler's forceps using a slit lamp for magnification. When, what, and where to culture are judgement calls. In small infected areas where the specimen may be sparse, an adequate culture is probably more important than a smear. The complications of perforation and denuding an intact epithelium may endanger a favorable prognosis.

HSV multinucleated epithelial cell
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Aqueous and vitreous specimens
The staining of aqueous and/or vitreous specimens is also diagnostic in detecting the appearance of bacteria, fungi, and the inflammatory response. ONLY TRAINED OPHTHALMOLOGISTS SHOULD OBTAIN INTRAOCULAR SPECIMENS. Aqueous and vitreous specimens should be placed directly on the slides from the tapping syringe and needle. The sample SHOULD NOT be smeared over the entire slide like a blood smear. This makes examining the slide more difficult. Slides are air-dried, fixed, stained, and examined. Intraocular specimens awaiting transport to the laboratory are quite stable at room temperature.

Pigment granules from vitreous
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