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2015 OMIG Abstract 18

The effect of light exposure on the efficacy and safety of Amphotericin B in
Optisol-GS corneal storage media

Katherine Duncan1, Jeff Parker2, Caroline Hoover3 and Bennie Jeng
1Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine
2University of Maryland Pathology Associates, PA.
3SightLife, Seattle, WA

In contrast to the corneal storage medium used in Europe, the most commonly used corneal storage medium in the US, Optisol-GS, does not contain an antifungal additive. Given the increasing rate of postkeratoplasty fungal infection, we sought to find a concentration of antifungal additive that would be safe and efficacious in eliminating fungal contaminants from Optisol-GS. Because amphotericin B is degraded by light, we also sought to determine what effect light exposure has on its efficacy and safety.

Vials of Optisol-GS were supplemented with Amphotericin B at 3 concentrations and then inoculated with C. albicans. Samples from each vial were plated on days 2, 7, and 14 and fungal colony counts were performed.

To assess for toxicity of amphotericin B, donor corneas were placed in Optisol-GS supplemented with Amphotericin B. All corneas were evaluated for endothelial cell density and % intact epithelium on day 0, 7 and 14. Half of the Optisol-GS vials and donor corneas were kept in light protected conditions.

There was no growth of C. albicans in any amphotericin B supplemented vials on days 7 and 14. Minimal growth was observed at the lower concentrations on day 2 only. There was no significant difference in intact epithelium and endothelial cell density between the amphotericin B supplemented and control corneas.  There was no difference in our data between the light exposed and light protected conditions.

Our study confirms the antifungal efficacy and safety of amphotericin B in Optisol-GS. Consideration should be given to the addition of an antifungal to the corneal storage process in the US.

Grant Support: 2014 Richard Lindstrom/EBAA Research Grant

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