          |
 |
2016 Agenda and Abstracts | < Previous | Next > 2016 OMIG Abstract 2 Sensitivity and Specificity of Laser In Vivo Confocal Microscopy for Diagnosis of Fungal Keratitis Purpose: In Vivo Confocal Microscopy (IVCM) is a non-invasive imaging modality which provides high-resolution images of the cornea. Previously, IVCM has been used for a rapid diagnosis of causative organisms in patients with acanthamoebal and fungal corneal infections. This study was designed to determine sensitivity and specificity of laser IVCM for detection of fungal elements in patients with proven fungal keratitis and to evaluate the effect of the observer’s imaging experience on these parameters. Methods: This study included 61 patients with acute infectious keratitis. The etiology of infection was proven to be fungal in 27 and bacterial (as controls) in 34 based on culture results and/or response to specific therapy. All patients had a full-thickness corneal imaging by laser IVCM (Heidelberg Retina Tomograph 3 with Rostock Cornea Module). To determine the sensitivity and specificity of IVCM for detection of fungal elements, the IVCM images were evaluated by 4 independent observers, including 2 IVCM experts and 2 non-expert cornea specialists. All observers were masked to the clinical data of patients. Results: Mean number of images analyzed per eye was 894 ± 350 (range, 288-1955). For detecting fungal elements by expert observers, IVCM had a mean sensitivity of 67% and a mean specificity of 84%. For trainee observers, the mean sensitivity and specificity were 52% and 84%, respectively. Conclusions: When used without clinical data, although IVCM has a high specificity for diagnosing fungal keratitis, its sensitivity is moderate and is dependent on the level of clinician’s experience with IVCM imaging. These should be considered in using IVCM for evaluating patients with infectious keratitis. Financial Support: None 2016
Agenda and Abstracts | < Previous | Next >
|
|
