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2018 Agenda and Abstracts | < Previous Next >

2018 OMIG Abstract

Optisol Supplemented with 0.255 mcg/ml Amphotericin B Does Not Eliminate Yeast at 5 C

Devin Tran, Deepinder Dhaliwal, Vishal Jhanji, RoheenaKamyar, Regis P. Kowalski
University of Pittsburgh Medical Center (UPMC), The Charles T. Campbell Eye Microbiology Laboratory, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA


Purpose: Fungal infections in lamellar keratoplasty is a growing concern. Optisol-GS does not contain an antifungal and supplementation with 0.255 g/ml has been considered. This study tests the ability of 0.255 g/ml Amphotericin B (AmpB) in Optisol-GS to eliminate yeast contamination of corneal tissue.

Methods: Three isolates of Candida albicans, one of C. parapsilosis, and one of C. glabratawere tested in Optisol with and without AmpB. Corneoscleral rims stored at -80OC were thawed and placed in 10 multiwell plates (4 per plate). The rims were inoculated with 4 respective loads of yeast: 0, 101, 102, and 103 colony forming units in two sets of 5 for five yeasts. One set was filled with Optisol plus AmpB and the other with Optisol only. All 10 plates were incubated at 5C for 48 hours. After 48 hours, all corneal rims were placed into 10 ml of yeast extract peptone dextrose medium (YPD); a swab culture of each well was plated onto Sabouraud plates; and all plates with the remaining Optisol were incubated at 30C. Yeast growth was monitored for 10 days. Minimum inhibitory (MIC) and bactericidal (MBC) concentrations were determined.

Results: All corneoscleral specimens were positive regardless of fungal load or presence of AmpB. All controls remained negative. MICs and MBCs were equivalent and ranged between 0.5 to 2.0 g/ml.

Conclusion: Amphotericin B at a concentration of 0.255 g/ml in Optisol-GS at 5C over 48 hours does not sterilize corneal specimens contaminated with yeast.

Disclosure: N


2018 Agenda and Abstracts | < Previous Next >