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2019 OMIG Abstract

Metagenomic Deep Sequencing for the Diagnosis of Corneal and
External Disease Infections

Gerami D. Seitzman, MD1,2, Armin Hinterwirth1, Lina Zhong1, Susie Cummings1, Cindi Chen1,
Todd H. Driver1,2, Michele D. Lee1,2, and Thuy Doan1,2
1Francis I. Proctor Foundation, University of California San Francisco, San Francisco, CA; 2Department of Ophthalmology, University of California, San Francisco, CA, USA

Purpose: To compare the performance of Metagenomic Deep Sequencing (MDS) to present gold standard microbiologic testing for the diagnosis of infections of the cornea, sclera, and conjunctiva.

Methods: Retrospective clinical case-series.

Results: All organisms identified by culture or PCR were also detected by MDS. MDS was able to identify parasitic, fungal, bacterial, and viral infections. The mean time from disease onset to gold standard diagnosis was 37 days (0 to 125 days, n=9). When atypical organisms were the cause of the infection (fungal or parasitic), the length of time to diagnosis further increased to 62 days (25 to 125 days, n=5).

Conclusion: For the diagnosis of corneal, scleral, and conjunctival infections, this proof-concept case series demonstrates MDS can replicate the identification of causative organisms as determined by current gold standard microbiologic testing. Especially with atypical infections, MDS has potential to be more efficient and economical.

Disclosure: C: (GDS, Dompé)

Grant and Financial Support: Research to Prevent Blindness Career Development Award (T.Doan); National Eye Institute of the National Institutes of Health under Award Number K08EY026986 (T.Doan); and the Huang Pacific Foundation (T.Doan).



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