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2003
OMIG, Abstract 23
OMIG
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Expression
Profile of Transcriptional Regulators in Cornea in a Mouse Model
of Infectious Keratitis
Joon Young Hyon, Stacey Hose, Debasish Sinha, Terrance P. O’Brien.
Ocular Microbiology Laboratory, The Wilmer Eye Institute, Johns
Hopkins University School of Medicine, Baltimore, Maryland, USA
Purpose:
From the unpublished SAGE data of normal mouse cornea, IRF-1 and
IRF-7 we shown to be up regulated during development. This study
was to investigate the role of transcriptional factors in immune
response against Pseudomonas aeruginosa keratitis in an experimental
mouse model.
Methods: BALB/c mice were topically inoculated
with 5-uI bacterial suspension containing 1.0 x 106 CFU of Pseudomonas
aeruginosa after scarifying the cornea with three horizontal scratches.
Normal corneas and scarified corneas without inoculation served
as controls. IRF-1, IRF-7, and iNOS expression was analyzed by Western
blot assay at 24 hours after the infection. NOS activity was measured
by the ability of tissue homogenate to convert [U-14C]arginine to
[U-14C]citrulline.
Results: Protein expression of IRF-I and lRF-7
increased in corneas infected with Pseudomonas compared to normal
or wounded cornea. Protein expression of iNOS and NOS enzyme activity
also increased in infected cornea. Control corneas and scarified
corneas had negligible amounts of NOS activity.
ConcIusions: IRF-I and IRF-7 play an important
role in regulating iNOS induced immune response against Pseudomonas
aeruginosa keratitis in mice. Further studies to define the precise
role of these regulatory factors to develop potential interventional
strategies for therapy of infectious keratitis are warranted.
OMIG
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