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OMIG Main Page | 2005 Abstracts | < Previous | Next > Biofilm Formation by S. epidermidis on Intraocular Lens Material S Kobayakawa1, Y Okajima1, A Tuji2, T Tochikubo1. 1Department of Ophthalmology, Toho U., Tokyo, Japan.. 2Department of infection control and prevention. School of Nursing, Faculty of Medicine, Toho U., Tokyo, Japan. School of Nursing, Faculty of Medicine, Toho U., Tokyo, Japan. Purpose: As biofilms on intraocular lens (IOL) have played an important role in postoperative endophthalmitis, the aim of this study was to compare the ability of Staphylococcus epidermidis to form biofilms on various IOL materials. Methods: Two S. epidermidis strains, ATCC 12228 (nonbiofilm-producer) and ATCC 35984 (biofilm-producer), were used to seed biofilms. Biofilms were cultivated on sample disks (6.0mm dia. X 1.0mm) of various (silicone, polymethylmethacrylate, acrylic) IOL materials in Tryptic Soy Broth with glucose. Biofilms were stained with crystal violet (CV) and the optical density ofCV extracted from stained biofilms used as an index of the extent ofbiofilm formation. Bacterial population within biofilms was determined following homogenization. Biofilms were also examined by scanning electron microscopy (SEM). Results: ATCC 35984, biofilm-producer strain, showed greater biofilm formation than ATCC 12228 (nonbiofilm-producer) on all materials tested. Biofilm on silicone was the least amount ofCV-stainable biofilm among the three materials in both strain (p<0.05). There were significant differences in the bacterial population between acrylic and another material at 24 hours incubation in both strain (p<0.05). Also, there were significant differences in SEM observation score between acrylic and another material through 72 hours (p<0.05). Conclusions: Among the three IOL materials tested, S. epidermidis biofilms formed more readily on acrylic compared to another material. Biofilm producer strain showed the greater biofilm formation. An understanding of bacteria-polymer interactions may be important in preventing lOL-associated intraocular infections. OMIG Main Page | 2005 Abstracts | < Previous | Next >
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