2005 OMIG, Abstract 13

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Prevention of Biofilm Formation by S. epidermidis on Intraocular Lens Material

Y Okajima¹, S Kobayakawa¹, A Tuji², T Tochikubo¹.

¹Department of Ophthalmology, Toho U., Tokyo, Japan. ²Department of infection control and prevention, School of Nursing, Faculty of Medicine, Toho U., Tokyo, Japan

Purpose: As biofilms on intraocular lens (IOL) have played an important role in postoperative endophthalmitis, the aim of this study was to compare the preventive ability of Staphylococcus epidermidis to form biofilms on two hydrophilic IOL materials.

Methods: Two S. epidermidis strains, ATCC 12228 (nonbiofilm-producer) and ATCC 35984 (biofilm-producer), were used to seed biofilms. Two hydrophilic IOL material, MFC [2-methacryloyk)xyethyl phosphorylcholineJ surface-modified acrylic and hydrogel (Meridian, HP60M, Bausch&Lomb) were used. Acrylic material was used as control. Biofilms were cultivated on sample disks of three various IOL materials in Tryptic Soy Broth with glucose. Biofilms were stained with crystal violet (CV) and the optical density of CV extracted from stained biofilms used as an index of the extent ofbiofilm formation. Bacterial population within biofilms was determined following homogenization. Biofilms were also examined by scanning electron microscopy (SEM).

Results: ATCC 35984 showed greater biofilm formation than ATCC 12228 on both hydrophilic IOL materials. CV-stainable biofilm and bacterial population on MFC surface-modified acrylic were less than those on acrylic through 48 hours incubation in both strain (p<0.05). There was no significant difference in the biofilm formation and the bacterial population within biofilms between hydrogel and acrylic material after 24 hours. However, after 48 and 72 hours incubation, hydrogel supported the less amount ofbiofilm formation compared to acrylic (p<0.05). There were significant differences in SEM observation score between MFC surface-modified acrylic and acrylic material through 72 hours (p<0.05).

Conclusions: Hydrophilic IOL materials have a preventive effect for biofilm formation by S. epidermidis. MFC surface modified acrylic was more effective for biofilm formation than hydrogel material at the initial 24 hours incubation. An understanding of bacteria-polymer interactions may be important in preventing lOL-associated intraocular infections.

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