Ocular Microbiology and Immunology Group
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2016 OMIG Abstract 9

Time-Kill Comparison of Povidone Iodine to Hypochlorous Acid Against Endophthalmitis Isolates of Staphylococci
Klocek MS, Mammen A, Dhaliwal DK, Kowalski RP
The Charles T. Campbell Laboratory, Ophthalmology, University of Pittsburgh, Pittsburgh, PA

Purpose: We compared the antibacterial activity of povidone iodine (PI) and hypochlorous acid (HA) against endophthalmitis isolates of Staphylococcus aureus and coagulase-negative staphylococcus using time-kill studies.

Methods: Time-kill studies of PI (5%), HA (0.008%), and HA (0.01%, Avenova), were conducted in duplicate against methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylcoccus aureus (MRSA), and coagulase-negative Staphylococcus (CNS) at time points 1, 2, 10, and 30 minutes. Each antiseptic was inoculated to a final bacterial concentration of 106 CFU/mL. The main outcome measure was based on a bactericidal decrease in colony counts units (CFU) (3 log10 or 99.9% decrease).

Results: At 1 minute, HA (0.008% and 0.01%) were more bactericidal than PI (5%) for decreasing the CFU of MRSA, MSSA, and CNS (p=0.045, Fisher’s Exact Test). At 2 minutes, the bactericidal effect was equivalent for PI (5%) and HA (0.008% and 0.01%) against the three Staphylococcal groups. The bactericidal effects of HA 0.008% and HA 0.01% were equivalent at all time points against all three Staphylococcal groups.

Conclusions: Povidone iodine is the current gold standard for endophthalmitis prophylaxis. This in vitro study supports a 2 minute contact time between Staphylococci and PI for a bactericidal effect. The contact time between HA (0.008% and 0.01%) and Staphylococci for a bactericidal effect appears to be reduced to 1 minute.

Disclosure Code: None

2016 Agenda and Abstracts | < Previous | Next >