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2019 OMIG Abstract

Rose Bengal Photodynamic Antimicrobial Therapy Safety Study. Pilot study

Jaime D. Martinez, MD1,2, Esdras Arrieta, MD2, Andrea Naranjo, MD1,2, Pedro Monsalve, MD1,
Alejandro Arboleda, MD2, Heather Durkee, MS2, Mariela C. Aguilar, PhD2, Sander R. Dubovy, MD1,
Harry W. Flynn Jr, MD1, Darlene Miller, DHSc2, Guillermo Amescua, MD1,2,
Jean-Marie Parel, PhD, FARVO1,2
1Anne Bates Leach Eye Center and 2Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL

To evaluate corneal changes on healthy New Zealand White (NZW) rabbits after rose bengal photodynamic antimicrobial therapy (RB-PDAT) treatment.

Methods: Twelve NZW rabbits were divided into four groups, each with three rabbits. Group 1: central 8 mm circle corneal de-epithelization (CDE), Group 2: CDE and 0.2% rose bengal drops (every 3 minutes for 30 minutes) over the cornea, Group 3: CDE and green light exposure (5.4 J/cm2) for 15 minutes, and Group 4: CDE and 0.1% of rose bengal over the cornea with green light exposure (5.4 J/cm2) for 15 minutes. They were clinically examined for 2 weeks group 13 , and group 4 for 6 weeks. Follow-ups included fluorescein staining and pachymetry testing. A subset of three NZW rabbits were treated with 0.1% RB-PDAT and followed for 36 days with anterior segment optical coherence tomography (AS-OCT) using the fluorescence settings analysis. Histopathological analysis was performed in all eyes.

Results: Fluorescein staining showed that the epithelial defect healed on day three in Groups 1-4. There was a slight increase in central corneal thickness (CCT) measured on day seven, but no statistically significant difference compared to the pre-treatment CCT. Furthermore, no statistical difference in changes of the CCT measurements were observed, except in Group 4 where the mean pre-operative CCT was 329.3 11.3 m. After RB-PDAT in two NZW rabbits, the CCT increased at day three (mean change +26 2.8 m; P<0.05). However, we were unable to obtain CCT in one NZW rabbit from Group 4 on day 21 to day 35 due to <300 m CCT (pre-operative CCT was 321 m). In the subset of rabbits , AS-OCT showed evidence of a crosslinking demarcation line after seven days post-treatment in all NZW rabbits at a mean depth of 140.9 m (+/- 6.93) and RB fluorescence remained evident in all treated eyes throughout the 36 days of follow-up. Histopathology analysis did not show any changes on the cornea, lens, iris, and retina tissue compare to control group.

Conclusion: RB-PDAT using 0.1% rose bengal with 5.4 J/cm2 of green light exposure did not show adverse effect in corneal epithelial healing, endothelial dysfunction and histopathological results.

Disclosure: N:

Supported by Edward D. and Janet K. Robson Foundation, Florida Lions Eye Bank and the Beauty of Sight Foundation, Drs. KR Olsen, ME Hildebrandt, Raksha Urs and Aaron Furtado; NIH Center Grant P30EY14801, Research to Prevent Blindness, Henri and Flore Lesieur Foundation (JMP).



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