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2002 Ocular Microbiology and Immunology Group, Abstract 19

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The In Vitro Evaluation of the New Antimicrobial Agent N-Chlorotaurine (NCT) Against Ocular Isolates of Adenovirus.
EG Romanowski1, KA Yates1, B Teuchner2, M Nagl2, W Gottardi2, RP Kowalski1, FS Mah1, YJ Gordon1. 1The Charles T. Campbell Laboratory, University of Pittsburgh, Pittsburgh, PA, 2University of Innsbruck, Innsbruck,
Austria.

Purpose: To evaluate the in vitro efficacy of N-chlorotaurine (NCT), an endogenous microbicidal oxidant produced by stimulated human leukocytes, against multiple ocular isolates of adenovirus (Ad).

Methods: In duplicate trials, the direct inactivating activity of NCT was determined by incubating high titer stocks of clinical isolates of Ad1, Ad2, Ad3, Ad4, Ad5, Ad7a, Ad8, Ad19, and Ad37 ATCC with 2.5%, 1.0%, 0.5%, and 0.1% NCT and control media for 60 minutes at room temp. Standard plaque assays were performed on the reaction mixtures to determine the Ad titers after NCT or control treatment. Ad titers were Log converted and expressed as the mean ± sd pfu/ml.

Results:
Mean Ad Titer (Log10 pfu/ml)
Ad
Control
2.5% NCT
1% NCT
0.5% NCT
0.1% NCT
Ad1 8.49 ± 1.42 2.74 ± 0.00 4.93 ± 0.71 4.15 ± 0.24 5.64 ± 0.93
Ad2 9.81 ± 0.13 2.72 ± 3.84 4.14 ± 1.78 6.03 ± 0.72 6.48 ± 0.07
Ad3 7.51 ± 0.02 1.60 ± 2.26 2.58 ± 3.64 2.58 ± 3.64 5.09 ± 0.98
Ad4 10.0 ± 0.04 5.04 ± 0.48 5.56 ± 0.91 5.40 ± 0.91 7.44 ± 0.11
Ad5 9.15 ± 1.12 0.00 ± 0.00 3.35 ± 1.34 5.00 ± 0.81 6.57 ± 0.08
Ad7a 7.22 ± 0.80 0.00 ± 0.00 3.42 ± 0.73 3.65 ± 1.53 5.23 ± 0.00
Ad8 4.43 ± 0.81 0.00 ± 0.00 1.44 ± 2.04 1.41 ± 1.99 3.44 ± 0.37
Ad19 10.1 ± 0.28 1.81 ± 2.56 4.72 ± 0.01 4.26 ± 1.46 6.92 ± 1.24
Ad37 7.48 ± 0.07 1.68 ± 2.38 1.72 ± 2.43 3.23 ±1.52 4.78 ± 0.11

Conclusions: NCT demonstrated concentration-dependent direct inactivation of all ocular Ad isolates tested. NCT concentrations of 0.5% or greater demonstrated at least a 3-Log reduction in the mean titers of all Ad isolates. Further study of NCT as an antiviral agent against adenovirus is warranted.

Support: NIH Grant EY08227, NIH Core Grant EY08098

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