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2004
OMIG, Abstract 12
OMIG
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Non-Invasive
Fluorophotometry Assessment of Corneal Epithelial Permeability After
Exposure to VigamoxTM or ZymarTM
BE McCarey, HF Edelhauser. Emory Eye Center, Atlanta, Georgia
Purpose:
To measure the rabbit corneal epithelial permeability non-invasively
following exposure to 4th generation fluoroquinolones antibiotic
solutions.
Methods:
The unanesthetized rabbit's lower eyelid was extended, such that,
the corneal surface was bathed in vivo for 3-minutes with BSS®,
Vigamox™, or Zymar™. Followed a 5-ml flush with BSS®,
the corneal surface was bathed in 0.075% sodium fluorescein for
3-minutes. Afterwards, corneal epithelial cell uptake of fluorescein
was assessed with the slit lamp and a fluorophotometer (OcuMetrics,
CA). The fluorophotometer was used to determine epithelial cell
layer permeability.
Results: There were three randomized groups (1)
OS treated with Vigamox™ and OD treated with BSS®,
n=10 rabbits, (2) OS treated with Zymar™ and OD treated with
BSS®, n=10 rabbits, and (3) OS treated with Vigamox™
and OD treated with Zymar™, n=10 rabbits. The BSS®
solution was used as a control solution. The corneal epithelial
cell layer permeability to sodium fluorescein (nm/sec) following
exposure to BSS® was 0.5241±0.208 nm/sec, to
Vigamox™ was 0.525±0.184 nm/sec, and Zymar™ was
6.315±2.454 nm/sec.
Paired t-tests of each treatment group were performed. In rabbits
treated with BSS® and Vigamox™ the paired t-test
had a p=0.9516. In rabbits treated with BSS® and Zymar™
the paired t-test had a p<0.0001. In rabbits treated with Zymar™
and Vigamox™ the paired t-test had a p<0.0001.
Conclusion: The epithelial permeabilities of corneas
exposed to either BSS® or the Vigamox™ were
statistically equivalent. By comparison, the epithelial permeabilities
of corneas exposed to either BSS® and Vigamox™
were statistically different from Zymar™. The Zymar™
caused a decrease in the epithelial cell layer barrier, i.e. a 12-fold
increase in epithelial permeability as compared to Vigamox™.
The change in permeability is comparable to the magnitude expected
from 0.005% benzalkonium chloride preservative. Zymar™ contains
0.005% benzalkonium chloride, whereas, BSS® and Vigamox™
are preservative free.
Supported
by Alcon Laboratories, and RPB, Inc.
Disclosure code: F for BEM & HPE
OMIG
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